Modification of simian virus 40 large tumor antigen by glycosylation
نویسندگان
چکیده
منابع مشابه
Simian virus 40 large tumor antigen: a "RNA binding protein"?
Simian virus 40 large tumor antigen was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified large tumor antigen, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP, and UMP. Furthermore, RNA fragments of approximately 45 nucleotides could be isolated from large tumor antigen pur...
متن کاملStimulation of simian virus 40 late gene expression by simian virus 40 tumor antigen.
The early simian virus 40 (SV40) gene product, large tumor (T) antigen, is responsible for the initiation of viral DNA replication and the autoregulation of early gene expression through direct protein-DNA interactions. We investigated the role of T antigen in late viral gene expression, independent of its function in amplifying templates through DNA replication. SV40 DNA was transfected into B...
متن کاملComplex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.
Simian virus 40 large tumor antigen (T Ag) can be separated by sucrose gradient sedimentation into a rapidly sedimenting, maximally phosphorylated fraction and a slowly sedimenting, less phosphorylated fraction. The Mr 48,000 host tumor antigen (48,000 HTA, also called nonviral T Ag) is preferentially complexed with the maximally phosphorylated T Ag. Pulse-labeled T Ag sediments as a 5-6S monom...
متن کاملGeometry of the simian virus 40 large tumor antigen-DNA complex as probed by protease digestion.
I have mapped the regions of simian virus 40 (SV40)-encoded large tumor (T) antigen that remained associated with origin-containing SV40 DNA after digestion with various concentrations of Pronase E, a nonspecific protease. Immunoaffinity-purified, labeled T antigen was bound to SV40 DNA-cellulose and treated with Pronase E. A "core" region representing amino acids 140 to about 281 was identifie...
متن کاملStat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.
To investigate the role of Stat3 (signal transducer and activator of transcription-3) in neoplastic transformation by the Large Tumor antigen of Simian Virus 40 (TAg), murine fibroblasts were rendered deficient in Stat3 activity through expression of a Stat3-specific siRNA or a Cre-loxP recombination system. The results demonstrate that growth rate, formation of foci overgrowing a monolayer of ...
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ژورنال
عنوان ژورنال: Virology
سال: 1985
ISSN: 0042-6822
DOI: 10.1016/0042-6822(85)90250-8